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Image Search Results
Journal: Frontiers in physiology
Article Title: Exogenous Wnt1 Prevents Acute Kidney Injury and Its Subsequent Progression to Chronic Kidney Disease.
doi: 10.3389/fphys.2021.745816
Figure Lengend Snippet: FIGURE 2 | In vivo expression of exogenous Wnt1 before IR reduces tubular cell apoptosis and NF-κB activation in AKI mice. (A) Representative micrographs show TUNEL-positive cells in different groups as indicated. Arrows indicate positive staining. Scale bar, 100 µm. (B) Graphic presentation shows the TUNEL-positive cells per high power field (HPF) in different groups as indicated. (C–F) Representative Western blot analyses show renal expression of FasL, p53, and Bax in different groups as indicated. *P < 0.05 versus Sham controls; †P < 0.05 versus IRI injected with pcDNA plasmids (n = 5–6). (G) Representative Western blot of p65 and p-p65 proteins in different groups as indicated.
Article Snippet: Primary antibodies included rabbit polyclonal anti-fibronectin (F3648; Sigma-Aldrich), mouse monoclonal anti-β-catenin antibody (610154; BD Transduction Laboratories), mouse monoclonal anti-α-SMA antibody (A2547; Sigma-Aldrich), rabbit polyclonal anti-Wnt1 (ab15251; Abcam), mouse anti-α-tubulin (T9026; Sigma-Aldrich), mouse antiPAI-1 antibody (AF3828;R&D Systems), anti-active β-catenin (#05–665; EMD Millipore),
Techniques: In Vivo, Expressing, Activation Assay, TUNEL Assay, Staining, Western Blot, Injection
Journal: Frontiers in physiology
Article Title: Exogenous Wnt1 Prevents Acute Kidney Injury and Its Subsequent Progression to Chronic Kidney Disease.
doi: 10.3389/fphys.2021.745816
Figure Lengend Snippet: FIGURE 8 | Exogenous Wnt1 protects tubular cells against apoptosis induced by hypoxia-reoxygenation (H/R) injury in vitro. (A) Representative FACS analyses show that Wnt1 inhibited hypoxia/reoxygenation (H/R)-induced cell apoptosis. HKC-8 cells were pre-expressed with exogenous Wnt1, followed by incubation in hypoxic condition for 48 h and then reoxygenation for 2 h. (B) Graphic presentation shows the percentage of apoptotic cells in different groups as indicated. The PE-labeled Annexin V-positive cells were counted by flow cytometry. *P < 0.05 versus controls; †P < 0.05 versus H/R (n = 3). (C) Representative Western blot analyses show protein expression of FasL, p53, Bax, and Parp-1 in HKC-8 cells. (D–G) Graphic presentations show the relative abundances of FasL, p53, Bax, and Parp-1 in HKC-8 cells. *P < 0.05 versus controls; †P < 0.05 versus H/R (n = 3).
Article Snippet: Primary antibodies included rabbit polyclonal anti-fibronectin (F3648; Sigma-Aldrich), mouse monoclonal anti-β-catenin antibody (610154; BD Transduction Laboratories), mouse monoclonal anti-α-SMA antibody (A2547; Sigma-Aldrich), rabbit polyclonal anti-Wnt1 (ab15251; Abcam), mouse anti-α-tubulin (T9026; Sigma-Aldrich), mouse antiPAI-1 antibody (AF3828;R&D Systems), anti-active β-catenin (#05–665; EMD Millipore),
Techniques: In Vitro, Incubation, Labeling, Cytometry, Western Blot, Expressing